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Comparative Study
. 1992 Mar;30(3):628-32.
doi: 10.1128/jcm.30.3.628-632.1992.

Species-specific identification of and distinction between Borrelia burgdorferi genomic groups by using 16S rRNA-directed oligonucleotide probes

R T Marconi  1 , L Lubke, W Hauglum, C F Garon
Affiliations
Comparative Study

Species-specific identification of and distinction between Borrelia burgdorferi genomic groups by using 16S rRNA-directed oligonucleotide probes

R T Marconi et al. J Clin Microbiol. 1992 Mar.

Abstract

Examination of a number of previously published aligned Borrelia 16S rRNA sequences revealed the presence of regions which could serve as oligonucleotide probe targets for both species-specific identification of Borrelia burgdorferi and distinction between genomic groups. Total cellular RNA isolated from Borrelia cultures was used in slot blot analysis. Radiolabeled oligonucleotides designed to hybridize to specific 16S rRNA targets were used as probes. These probes allowed for both species-specific identification and genomic group typing of B. burgdorferi.

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References

    1. J Bacteriol. 1992 Jan;174(1):241-4 - PubMed
    1. J Exp Med. 1982 Nov 1;156(5):1297-311 - PubMed
    1. J Clin Microbiol. 1990 Jun;28(6):1089-93 - PubMed
    1. J Clin Microbiol. 1990 Jul;28(7):1673-5 - PubMed
    1. Science. 1990 Sep 21;249(4975):1420-3 - PubMed

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