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. 1992 May;273(3):271-80.
doi: 10.1016/0921-8777(92)90089-l.

Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada

K Angelis  1 , J Bríza, J Satava, I Skákal, J Velemínský, J Vlasák, K Kleibl, G P Margison
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Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada

K Angelis et al. Mutat Res. 1992 May.

Abstract

The protein coding region of the E. coli gene ada has been transferred to tobacco plants by a leaf disc transformation procedure involving an Agrobacterium tumefaciens Ti plasmid. Transformed plants were shown to be transgenic for the ada message and had increased levels of O6-alkylguanine DNA alkyltransferase activity. The N-methyl-N-nitrosourea- or taurinechlorethylnitrosourea-induced inhibition of growth of calluses or of cells in suspension was considerably lower in ada-transformed than in non-transformed plants. This indicates that O6-alkylguanine, O4-alkylthymine or phosphotriesters are growth-inhibitory lesions in tobacco.

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