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. 1992 Jun;60(6):2488-92.
doi: 10.1128/iai.60.6.2488-2492.1992.

Localization of two epitopes recognized by monoclonal antibody PCG-4 on Clostridium difficile toxin A

S M Frey  1 , T D Wilkins
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Localization of two epitopes recognized by monoclonal antibody PCG-4 on Clostridium difficile toxin A

S M Frey et al. Infect Immun. 1992 Jun.

Abstract

The toxin A gene of Clostridium difficile contains a 2.5-kb region encoding a series of contiguous repeating units located at the COOH terminus of the molecule. We previously showed that the monoclonal antibody (MAb) PCG-4, which neutralizes the enterotoxic activity of toxin A, binds to epitopes located within these repeating units. In the present study, we subcloned a series of fragments from this portion of the gene. The recombinant peptides expressed from the gene fragments were examined for reactivity with MAb PCG-4 to identify the epitopes involved in binding. Our results showed that MAb PCG-4 recognizes epitopes in amino acid residues 2097 through 2141 and amino acid residues 2355 through 2398.

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