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. 1961 Aug;10(4):467-74.
doi: 10.1083/jcb.10.4.467.

The direct isolation of the mitotic apparatus

D MAZIA, J M MITCHISON, H MEDINA, P HARRIS

The direct isolation of the mitotic apparatus

D MAZIA et al. J Biophys Biochem Cytol. 1961 Aug.

Abstract

A method for isolating the mitotic apparatus from dividing sea urchin eggs without the use of ethyl alcohol or of detergents is described. In the present method, the eggs are dispersed directly in a medium containing 1 M (to 1.15 M) sucrose, 0.15 M dithiodiglycol, and 0.001 M Versene at pH 6, releasing the visibly intact mitotic apparatus. The method is designed for studies of enzyme activities, lipid components, and the variables affecting the stability of the apparatus.

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