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. 1976 Dec 1;159(3):803-6.
doi: 10.1042/bj1590803.

Re-investigation of the effects of L-glutamine and L-asparagine on the Neurospora crassa NADP-specific glutamate dehydrogenase

J C Wootton, J P Kavanagh, A J Baron, M G Lovett

Re-investigation of the effects of L-glutamine and L-asparagine on the Neurospora crassa NADP-specific glutamate dehydrogenase

J C Wootton et al. Biochem J. .

Abstract

L-Glutamine, when purified free of traces of NH4+ present in solution, does not act as an alternative substrate to NH4+ for the NADP-specific glutamate dehydrogenase of Neurospora. L-Glutamine interferes with detection of small quantities of NH4+ by Nessler's reagent. L-Asparagine is not an alternative substrate to NH4+ for this enzyme.

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