doi: 10.1038/358123a0.
RNA substrate binding site in the catalytic core of the Tetrahymena ribozyme
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- PMID: 1377367
- DOI: 10.1038/358123a0
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RNA substrate binding site in the catalytic core of the Tetrahymena ribozyme
Nature.
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Abstract
In catalysis by group I introns, the helix (P1) containing the RNA cleavage site must be positioned next to the guanosine binding site. We have identified a conserved adenine in the catalytic core that contributes to the stability of this arrangement and propose that it accepts a hydrogen bond from a specific 2'-OH in P1. Such base-backbone tertiary interactions may be generally important to the organization of RNA tertiary structure.
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