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. 1992 Jul;11(7):2457-63.
doi: 10.1002/j.1460-2075.1992.tb05310.x.

The aerolysin membrane channel is formed by heptamerization of the monomer

H U Wilmsen  1 , K R Leonard, W Tichelaar, J T Buckley, F Pattus
Affiliations

The aerolysin membrane channel is formed by heptamerization of the monomer

H U Wilmsen et al. EMBO J. 1992 Jul.

Abstract

The cytolytic toxin aerolysin has been found to form heptameric oligomers by SDS-PAGE electrophoresis, STEM mass measurements of single oligomers and image analysis of two-dimensional membrane crystals. Two types of crystal, flat sheets and long regular tubes, have been obtained by reconstitution of purified protein and Escherichia coli phospholipids. A noise-filtered image of the best crystalline sheets reveals a structure with 7-fold symmetry containing a central strongly stain-excluding ring that encircles a dark stain-filled channel 17 A in diameter. The ring is surrounded by seven arms each made up of two unequal sized domains. By combining projected views and side-views, a simplified model of the aerolysin channel complex has been constructed. The relevance of this structure to the mode of action of aerolysin is discussed.

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