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. 1992 Feb;30(1-2):27-33.
doi: 10.1007/BF00554425.

Recovery of mitochondrial DNA from blood leukocytes using detergent lysis

G L Lindberg  1 , C M Koehler, J E Mayfield, A M Myers, D C Beitz
Affiliations

Recovery of mitochondrial DNA from blood leukocytes using detergent lysis

G L Lindberg et al. Biochem Genet. 1992 Feb.

Abstract

Mitochondrial DNA (mtDNA) was isolated from leukocytes contained in whole blood of cattle. Leukocyte membranes except the nuclear envelope were solubilized in a buffer that contained 1% Triton X-100. After sedimentation of cell nuclei, mtDNA was purified from the cell lysate by organic solvent extraction and ethanol precipitation. Approximately 5 micrograms of mtDNA was recovered from 400 ml of whole blood, a quantity sufficient for routine DNA cloning procedures or for detailed restriction mapping studies. mtDNA isolated with this method is a suitable substrate for several DNA-modifying enzymes. Thus, preparation of mtDNA from blood by detergent lysis provides a noninvasive alternative to tissue biopsy for characterization of mitochondrial genotypes in studies of evolutionary genetics and population dynamics.

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