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. 1992 Sep;13(3):380-6.

A nonradioactive micro-assay for released reverse transcriptase activity of a lentivirus

R F Cook  1 , S J Cook, C J Issel
Affiliations
  • PMID: 1382469

A nonradioactive micro-assay for released reverse transcriptase activity of a lentivirus

R F Cook et al. Biotechniques. 1992 Sep.

Abstract

A nonradioactive micro-assay procedure for detection of released reverse transcriptase activity from cells infected with equine infectious anemia virus is described. This procedure utilizes biotinylated-dUTP in conjunction with a streptavidin-alkaline phosphatase conjugate. Detection of alkaline phosphatase is by autoradiography of the chemiluminescence produced during enzymatic dephosphorylation of Lumi Phos 530. This method, as with reverse transcriptase micro-assays employing 32P-labeled nucleotides, is suited to the processing of numerous samples, while having the advantages of safety and stability normally associated with nonradioactive methods of detection. Sensitivity is comparable to a reverse transcriptase micro-assay using 32P-dTTP.

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