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. 1992 Oct;174(20):6674-7.
doi: 10.1128/jb.174.20.6674-6677.1992.

Site-specific integration of the Haemophilus influenzae bacteriophage HP1: location of the boundaries of the phage attachment site

M A Hauser  1 , J J Scocca
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Site-specific integration of the Haemophilus influenzae bacteriophage HP1: location of the boundaries of the phage attachment site

M A Hauser et al. J Bacteriol. 1992 Oct.

Abstract

Plasmids containing DNA segments from the attachment region of phage HP1 were constructed and tested for the ability to replace the phage attachment site substrate in site-specific recombination reactions. The distance separating the boundaries of the functional site was 418 bp. Replacements within the 11-residue segment 5'-GGCGGTTATCG at the left boundary or within the 12-residue segment 5'-GGATTTTTTGAA at the right boundary abolished substrate activity. A segment of the 418-residue sequence preserves the integrity of an operon of three Haemophilus influenzae tRNA genes after HP1 insertion within the coding sequence.

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References

    1. Nucleic Acids Res. 1978 May;5(5):1445-64 - PubMed
    1. Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:429-37 - PubMed
    1. Annu Rev Biochem. 1989;58:913-49 - PubMed
    1. J Biol Chem. 1988 Mar 15;263(8):3554-7 - PubMed
    1. Nucleic Acids Res. 1988 Oct 25;16(20):9687-705 - PubMed

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