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. 1992 Oct;30(10):2717-21.
doi: 10.1128/jcm.30.10.2717-2721.1992.

Development of cDNA probes for typing group A bovine rotaviruses on the basis of VP4 specificity

A V Parwani  1 , B I Rosen, M A McCrae, L J Saif
Affiliations

Development of cDNA probes for typing group A bovine rotaviruses on the basis of VP4 specificity

A V Parwani et al. J Clin Microbiol. 1992 Oct.

Abstract

Dot and Northern (RNA) blot hybridization assays were developed for the P typing of group A bovine rotaviruses (BRV) by using cDNA probes prepared from gene segment 4. The probes were prepared by polymerase chain reaction amplification of hyperdivergent regions (nucleotides 211 to 686) of BRV strain UK, IND, NCDV, and Cr VP4 cDNA by using specific oligonucleotide primers. The probes were P type specific (VP4) and exhibited little or no cross-reactivity with double-stranded RNA from heterologous rotavirus P types. Our studies indicate that at least three P types, as defined by polymerase chain reaction-derived VP4 gene probes from the UK, NCDV, and Cr strains, exist among the seven BRV isolates tested.

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References

    1. J Clin Microbiol. 1992 Jan;30(1):85-92 - PubMed
    1. Virology. 1982 Sep;121(2):288-95 - PubMed
    1. J Clin Microbiol. 1991 Sep;29(9):2043-6 - PubMed
    1. J Clin Microbiol. 1990 Feb;28(2):276-82 - PubMed
    1. J Virol. 1990 Aug;64(8):4021-4 - PubMed

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