Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Oct;35(10):1218-26.
doi: 10.1002/art.1780351015.

Antibodies in rabbits immunized with cationized IgG react with histones H3 and H4

T Suzuki  1 , R W Burlingame, F Cavalot, G Andres, S Kashiwazaki, E M Tan
Affiliations

Antibodies in rabbits immunized with cationized IgG react with histones H3 and H4

T Suzuki et al. Arthritis Rheum. 1992 Oct.

Abstract

Objective: Rabbits immunized with cationized rabbit IgG develop antinuclear antibodies. This study was aimed at identifying the reactive antigens.

Methods: Rabbits were immunized with homotypic or allotypic IgG that were physicochemically altered to produce positively charged dimethylpropanamide (polycationic) side chains.

Results: Seven of 11 rabbits injected with cationized IgG produced antinuclear antibodies detected by indirect immunofluorescence. By enzyme-linked immunosorbent assay, these were IgG antibodies reacting with histone (H3-H4)2 tetramer and with individual histone polypeptides H3 and H4. The antihistone antibodies were absorbed by cationized IgG but not by normal IgG.

Conclusion: Cationized IgG appears to possess antigenic determinants of sufficient similarity to produce antibody responses cross-reactive with histones H3 and H4.

PubMed Disclaimer

Publication types

LinkOut - more resources