Bacteriophage cloning and Escherichia coli expression of a human IgM Fab
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- PMID: 1385989
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Bacteriophage cloning and Escherichia coli expression of a human IgM Fab
Hum Antibodies Hybridomas.
1992 Apr.
Abstract
We have combined the molecular biology methods of the polymerase chain reaction and recombinant DNA cloning in bacteriophage lambda to express a human IgM Fab in Escherichia coli using genes derived from an Epstein-Barr virus transformed cell line. This method comprises three cDNA amplifications and a single cloning step, culminating in the stable overexpression of mammalian heterodimeric recombinant protein in a prokaryotic host.
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