Studies on the metabolic function of branched-chain volatile fatty acids, growth factors for ruminococci. I. Incorporation of isovalerate into leucine

Abstract

Allison, Milton J. (Dairy Cattle Research Branch, U. S. Department of Agriculture, Beltsville, Md.), M. P. Bryant, and R. N. Doetsch. Studies on the metabolic function of branched-chain volatile fatty acids, growth factors for ruminococci. I. Incorporation of isovalerate into leucine. J. Bacteriol. 83:523-532. 1962.-Ruminococcus flavefaciens strain C94, a cellulolytic rumen bacterium, requires either isobutyrate or isovalerate for growth. The organism was grown in the presence of C(14)-labeled isovalerate, and the metabolic fate of the labeled carbon was studied to obtain information on the functions of this growth factor. Radioactivity from isovalerate-1-C(14) and isovalerate-3-C(14) was found mainly in the protein and lipid fractions of the cells. The C(14) in protein was all in leucine, indicating that a function of isovalerate was to serve as a carbon skeleton for leucine synthesis. As C(14) in leucine synthesized from isovalerate-1-C(14) was entirely in carbon 2, the intact isovalerate molecule was apparently incorporated into leucine. This is evidence that leucine was synthesized by a mechanism different from that previously demonstrated in other microorganisms.R. flavefaciens has a definite but limited ability to incorporate exogenous amino acids, including leucine. It incorporated 2% of the C(14) during growth in uniformly labeled (UL) C(14)-Chlorella protein hydrolyzate; Escherichia coli incorporated 37% of the label under similar conditions. In another experiment, a limited amount of exogenous leucine-2-C(14) was incorporated into protein of R. flavefaciens. The requirement for isovalerate was not replaced by dl-leucine or 2-ketoisocaproate. It is suggested that isovalerate or isobutyrate is required because R. flavefaciens has a limited ability to incorporate exogenous branched-chain amino acids and a limited ability to synthesize the isopropyl group found in these amino acids and in other components of the cell.