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. 1962 Mar 1;115(3):453-66.
doi: 10.1084/jem.115.3.453.

The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes

S BOYDEN

The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes

S BOYDEN. J Exp Med. .

Abstract

An in vitro technique is described for assessing the chemotactic activity of soluble substances on motile cells. Antibody-antigen mixtures when incubated (37 degrees C) in medium containing fresh (i.e. non-inactivated) normal rabbit serum exert a strong chemotactic effect on rabbit polymorphonuclear leucocytes. Results are described which indicate that, when antibody-antigen complexes are incubated (37 degrees C) in fresh serum, a heat-stable (56 degrees C) substance (or substances) is produced which acts directly as a chemotactic stimulus on the polymorphs. This heat-stable chemotactic substance is not produced when antibody-antigen complexes are incubated in serum which has been heated at 56 degrees C for 30 minutes.

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