Large scale purification of proteinases from Clostridium histolyticum filtrates

Abstract

Conklin, David A. (Walter Reed Army Institute of Research, Washington, D. C.), Marion E. Webster, Patricia L. Altieri, Sanford Berman, Joseph P. Lowenthal, and Raymond B. Gochenour. Large scale purification of proteinases from Clostridium histolyticum filtrates. J. Bacteriol. 82:589-594. 1961.-A method for the large scale preparation and partial purification of Clostridium histolyticum proteinases by fractional precipitation with ammonium sulfate is described. Conditions for adequate separation and purification of the delta-proteinase and the gelatinase were obtained. Collagenase, on the other hand, was found distributed in four to five fractions and little increase in purity was achieved as compared to the crude ammonium sulfate precipitates.