Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Sep;13(3):388-91.

Improved method for screening cDNA expression libraries for DNA-binding proteins

M C Harline  1 , J C Kandala, R D Sage, R V Guntaka, A DeAngelo
Affiliations
  • PMID: 1389172

Improved method for screening cDNA expression libraries for DNA-binding proteins

M C Harline et al. Biotechniques. 1992 Sep.

Abstract

The ability to successfully screen a lambda gt11 cDNA expression library for specific gene products that can bind to selected sequences of DNA depends on radioactive double-stranded DNA probes with high specific activity. We demonstrate here that probes labeled by the PCR are superior to probes made by the Klenow reaction. The use of these PCR-generated probes have facilitated our efforts to isolate recombinant phage containing putative DNA-binding gene products that recognized a 246-base pair transcriptional enhancer region of Rous sarcoma virus long terminal repeat.

PubMed Disclaimer

Publication types