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. 1962 Jun;13(3):437-43.
doi: 10.1083/jcb.13.3.437.

A new epoxy embedment for electron microscopy

J A FREEMAN, B O SPURLOCK

A new epoxy embedment for electron microscopy

J A FREEMAN et al. J Cell Biol. 1962 Jun.

Abstract

A new epoxy embedding mixture has been developed utilizing Maraglas 655 and Cardolite NC-513 with benzyldimethylamine (BDMA) as a curing agent. This epoxy mixture permits cellular preservation comparable to that obtained with Epon 812, ease of preparation of tissues, a wide range of miscibility, low viscosity, and, most important, ease of sectioning on a Porter-Blum microtome. In contrast to Epon-812-embedded tissues, Maraglas-Cardolite-embedded tissues can be sectioned in large dimensions with ease and consistent results without "chatter." No background granularity is detectable with high magnification study of Maraglas-Cardolite-embedded tissues. This epoxy is readily stained with lead hydroxide and is relatively stable in the electron beam.

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References

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