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. 1963 Jul;11(4):320-5.
doi: 10.1128/am.11.4.320-325.1963.

Fractionation of beta-glucosidases and related extracellular enzymes from Aspergillus niger

L H LI, K W KING

Fractionation of beta-glucosidases and related extracellular enzymes from Aspergillus niger

L H LI et al. Appl Microbiol. 1963 Jul.

Abstract

Industrial concentrates from Aspergillus niger culture filtrates were fractionated by ion-exchange and adsorption chromatography. Several other types of hydrolases were completely removed. Eight partially purified components were obtained. Using specific activity as an estimate of purification, one aryl-beta-glucosidase was purified 35-fold. Another component showed 147-fold purification using a viscosimetric assay with carboxymethylcellulose as substrate. The aryl-beta-glucosidase was distinctly more thermolabile than the carboxymethylcellulase.

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References

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