doi: 10.1007/BF00317931.
A ten-minute protocol for transforming Saccharomyces cerevisiae by electroporation
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- PMID: 1394517
- DOI: 10.1007/BF00317931
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A ten-minute protocol for transforming Saccharomyces cerevisiae by electroporation
Curr Genet.
1992 Oct.
Abstract
We present a simplified and rapid method for the transformation of yeast cells by electroporation. Stationary cells, scraped off the agar of Petri dish cultures stored in the refrigerator for up to 6 weeks, are suspended in sorbitol buffer, spun down by gentle centrifugation, transferred into the electroporation cuvette, and immediately subjected to transformation via electroporation. Transformation efficiency of this 10-min method, which does not require the preparation of cell cultures, is about 10% of the hitherto best performing transformation procedure using cells of defined growth phase.
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