Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Oct;22(4):335-6.
doi: 10.1007/BF00317931.

A ten-minute protocol for transforming Saccharomyces cerevisiae by electroporation

M Grey  1 , M Brendel
Affiliations

A ten-minute protocol for transforming Saccharomyces cerevisiae by electroporation

M Grey et al. Curr Genet. 1992 Oct.

Abstract

We present a simplified and rapid method for the transformation of yeast cells by electroporation. Stationary cells, scraped off the agar of Petri dish cultures stored in the refrigerator for up to 6 weeks, are suspended in sorbitol buffer, spun down by gentle centrifugation, transferred into the electroporation cuvette, and immediately subjected to transformation via electroporation. Transformation efficiency of this 10-min method, which does not require the preparation of cell cultures, is about 10% of the hitherto best performing transformation procedure using cells of defined growth phase.

PubMed Disclaimer

References

    1. Yeast. 1986 Sep;2(3):163-7 - PubMed
    1. Nucleic Acids Res. 1988 Jul 11;16(13):6127-45 - PubMed
    1. Biotechniques. 1988 Sep;6(8):742-51 - PubMed
    1. Methods Enzymol. 1991;194:182-7 - PubMed
    1. Biochem Biophys Res Commun. 1982 Jul 30;107(2):584-7 - PubMed

LinkOut - more resources