[Rapid diagnosis of pulmonary Pseudomonas aeruginosa infections by indirect immunofluorescent antibody]

Abstract

167 sputum specimens collected from patients with pulmonary infection were tested by IFA (Indirect immunofluorescent antibody-staining) with serogroup-specific monoclonal antibody to Pseudomonas aeruginosa, compared with quantitative sputum culture. The results showed the minimum concentration of bacteria detectable by IFA was 10 cfu/ml. 31(86.1%) were positive in 36 specimens with more than 10 cfu/ml of Pseudomonas aeruginosa. 22(100%) were positive in 22 specimens with more than 10 cfu/ml of Pseudomonas aeruginosa. In 127 specimens with a negative culture for Pseudomonas aeruginosa, 121(95.2%) were negative, 6(4.8%) were false-positive which could be identified by Gram's-staining. The type of Pseudomonas aeruginosa could also be identified within 3 hours.