Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1962 Nov;10(6):577-9.
doi: 10.1128/am.10.6.577-579.1962.

Ballistic disruption of Penicillium chrysogenum cells

P B HAMILTON, S G KNIGHT

Ballistic disruption of Penicillium chrysogenum cells

P B HAMILTON et al. Appl Microbiol. 1962 Nov.

Abstract

The use of glass beads in a high-speed mixing device to rupture organisms was applied to molds. The use of a mixer in which the propellor shaft enters from the top into a metal mixing chamber made it possible to immerse the whole device in a salt water and ice mixture so that the temperature of the glass-bead slurry could be kept below 5 C without difficulty. Mycelia, glass beads, and buffer in a 1:2:3 (w/w) ratio gave above 95% breakage in 15 min with Penicillium chrysogenum cells and in 4 min with Rhizopus nigricans. Some of the factors influencing breakage are discussed.

PubMed Disclaimer

References

    1. J Bacteriol. 1954 Apr;67(4):503-4 - PubMed
    1. Appl Microbiol. 1959 Sep;7:318-9 - PubMed
    1. J Bacteriol. 1957 Jul;74(1):79-86 - PubMed
    1. Plant Physiol. 1954 Sep;29(5):458-67 - PubMed
    1. Br J Exp Pathol. 1951 Apr;32(2):97-109 - PubMed

LinkOut - more resources