doi: 10.1159/000133393.
Segregation analysis reveals tight genetic linkage between the spontaneously arising neural tube defect gene splotch (Sp) and Pax-3 in an intraspecific mouse backcross
Affiliations
- PMID: 1395725
- DOI: 10.1159/000133393
Item in Clipboard
Segregation analysis reveals tight genetic linkage between the spontaneously arising neural tube defect gene splotch (Sp) and Pax-3 in an intraspecific mouse backcross
Cytogenet Cell Genet.
1992.
Abstract
Concurrent research has recently characterized Sp2H, a radiation induced mutation at the splotch (Sp) locus, and found alterations in the murine paired box gene, Pax-3, in homozygous Sp2H DNA. It was proposed that Sp and Pax-3 are the same gene. This report presents additional genetic evidence in support of this finding through linkage studies. Southern blot analysis of genomic DNAs from a panel of 125 intraspecific [(Sp/+ x CBA/J)F1-Sp x CBA/J] backcross mice reveals no crossover between Pax-3 and the spontaneously occurring splotch allele, Sp. This positions Pax-3 within 2.9 cM of the Sp locus (95% confidence interval) and suggests tight genetic linkage between the two marker genes.
Similar articles
-
Splotch (Sp2H), a mutation affecting development of the mouse neural tube, shows a deletion within the paired homeodomain of Pax-3.Cell. 1991 Nov 15;67(4):767-74. doi: 10.1016/0092-8674(91)90071-6. Cell. 1991. PMID: 1682057
-
The splotch-delayed (Spd) mouse mutant carries a point mutation within the paired box of the Pax-3 gene.Genomics. 1993 Aug;17(2):364-9. doi: 10.1006/geno.1993.1333. Genomics. 1993. PMID: 8406487
-
Analysis of the Pax-3 gene in the mouse mutant splotch.Genomics. 1993 Aug;17(2):355-63. doi: 10.1006/geno.1993.1332. Genomics. 1993. PMID: 8406486
-
Pax genes and neural tube defects in the mouse.Ciba Found Symp. 1994;181:103-13; discussion 113-7. doi: 10.1002/9780470514559.ch7. Ciba Found Symp. 1994. PMID: 7911756 Review.
-
Splotch locus mouse mutants: models for neural tube defects and Waardenburg syndrome type I in humans.J Med Genet. 1992 Mar;29(3):145-51. doi: 10.1136/jmg.29.3.145. J Med Genet. 1992. PMID: 1552554 Free PMC article. Review. No abstract available.
Cited by
-
A mutation within intron 3 of the Pax-3 gene produces aberrantly spliced mRNA transcripts in the splotch (Sp) mouse mutant.Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):532-6. doi: 10.1073/pnas.90.2.532. Proc Natl Acad Sci U S A. 1993. PMID: 8421686 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Medical
Molecular Biology Databases