RNA editing of atp6 transcripts from male-sterile and normal cytoplasms of rapeseed (Brassica napus L.)

Abstract

The complete cDNA sequence corresponding to the rapeseed atp6 gene transcript (coding for subunit 6 of F0-ATPase) has been determined by a method involving cDNA synthesis, using specific oligonucleotides as primers, followed by PCR amplification, cloning and sequencing of the amplification products. Only one modification, a C-to-U conversion, has been found when compared to the genomic mitochondrial DNA sequence. Comparison of the extent and frequency of RNA editing of the pol cytoplasmic male sterile (cms) atp6 transcript with those of normal atp6 transcript indicates that there is no variation between the editing status of the atp6 transcripts from pol cms and normal cytoplasms.