Continuous culture of ruminal microorganisms in chemically defined medium. I. Design of continuous-culture apparatus
- PMID: 13972781
- PMCID: PMC1057919
- DOI: 10.1128/am.10.6.580-582.1962
Continuous culture of ruminal microorganisms in chemically defined medium. I. Design of continuous-culture apparatus
Abstract
An apparatus is described which has been used for successful continuous culture of the ciliates from the rumen of cattle. Automatic control of feeding rate, pH, oxidation-reduction potential, temperature, stirring rate, aeration rate, salinity, and volume of the culture is provided for, using standard commercial equipment, whenever possible. The operation of this apparatus is described.
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