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. 1963 May;17(2):443-54.
doi: 10.1083/jcb.17.2.443.

Some observations on negatively stained mitochondria

W STOECKENIUS

Some observations on negatively stained mitochondria

W STOECKENIUS. J Cell Biol. 1963 May.

Abstract

A particulate component of mitochondria is described which is observed in negatively stained preparations of unfixed whole mitochondria or their fragments. It consists of a roughly spherical particle, approximately 85 A in diameter, found on the surface of the cristae and probably also on the side of the mitochondrial envelope which faces the matrix. It is attached to the underlying membrane by a narrow stalk 40 to 50 A long. The particle appears to be rather labile and it is destroyed or at least rendered unrecognizable by all conventional fixatives used in electron microscopy, especially OsO(4).

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References

    1. Cancer Res. 1951 Jan;11(1):1-22 - PubMed
    1. J Histochem Cytochem. 1953 Jul;1(4):188-211 - PubMed
    1. Acta Haematol. 1955 Jun;13(6):337-51 - PubMed

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