Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Aug;83(8):894-8.
doi: 10.1111/j.1349-7006.1992.tb01996.x.

Production of human immunoglobulin G reactive against human cancer in tumor-bearing mice with severe combined immunodeficiency reconstituted with human splenic tissues

T Furukawa  1 , M Watanabe, T Kubota, H Yamaguchi, T Teramoto, K Ishibiki, M Kitajima
Affiliations

Production of human immunoglobulin G reactive against human cancer in tumor-bearing mice with severe combined immunodeficiency reconstituted with human splenic tissues

T Furukawa et al. Jpn J Cancer Res. 1992 Aug.

Abstract

Splenic tissues derived from patients with gastric cancer were implanted into mice with severe combined immunodeficiency (SCID) and then the mice were challenged with COLO-205, a human colon cancer cell line. Production of human immunoglobulin G (IgG) reactive against the COLO-205 cells was detected by enzyme-linked immunosorbent assay in sera from the reconstituted and tumor-bearing SCID mice. The titers of the reactive IgG relative to total IgG in the sera of SCID mice began to increase from one week after implantation of the tumor cells, and became 10- to 100-fold higher than that in the donor's serum by 3-4 weeks. This model using implantation of human cancer cells in SCID mice reconstituted with human splenic tissues would facilitate further studies of human cancer immunology.

PubMed Disclaimer

References

    1. ) Bosma , G. C. , Custer , R. P. and Bosnia , M. J.A severe combined immunodeficiency mutation in the mouse . Nature , 301 , 527 – 530 ( 1983. ). - PubMed
    1. ) McCune , J. M. , Namikawa , R. , Kaneshima , H. , Shultz , L. D. , Lieberman , M. and Weissman , I. L.The SCID‐hu mouse: murine model for the analysis of human hematolymphoid differentiation and function . Science , 241 , 1632 – 1639 ( 1988. ). - PubMed
    1. ) Mosier , D. E. , Gulizia , R. J. , Baird , S. M. and Wilson , D. B.Transfer of a functional human immune system to mice with severe combined immunodeficiency . Nature , 335 , 256 – 259 ( 1988. ). - PubMed
    1. ) Duchosal , M. A. , Eming , S. A. , Fischer , P. , Leturcq , D. , BarbasIII , C. F. , McConahey , P. J. , Caothien , R. H. , Thornton , G. B. , Dixon , F. J. and Burton , D. R.Immunization of hu‐PBL‐SCID mice and the rescue of human monoclonal Fab fragments through combinatorial libraries . Nature , 355 , 258 – 262 ( 1992. ). - PubMed
    1. ) Kubota , T. , Yamaguchi , H. , Watanabe , M. , Yamamoto , T. , Takahara , T. , Takeuchi , T. , Furukawa , T. , Kase , S. , Kodaira , S. , Ishibiki , K. and Kitajima , M.High human IgG levels in severe combined immunodeficient mouse re‐constituted with human splenic tissues from patients with gastric cancer . Jpn. J. Cancer Res. , 83 , 300 – 303 ( 1992. ). - PMC - PubMed

LinkOut - more resources