Review
doi: 10.1016/0378-4347(92)80541-w.
Detection of the enzymatic activity of cytochrome P-450 enzymes by high-performance liquid chromatography
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- PMID: 1400829
- DOI: 10.1016/0378-4347(92)80541-w
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Review
Detection of the enzymatic activity of cytochrome P-450 enzymes by high-performance liquid chromatography
J Chromatogr.
.
Abstract
The reactions catalysed by the various cytochrome P-450 enzymes are reviewed with respect to the analysis of products by high-performance liquid chromatography (HPLC). Especially biotransformation reactions of purified cytochrome P-450 enzymes in a reconstituted system and in microsomes mainly of rat liver origin are considered. Emphasis is put on the specificity of product formation due to the individual isozymes of cytochrome P-450. It is shown that the presence of eight cytochrome P-450 isozymes can be monitored and determined by specific product formation after HPLC analysis, which is an important parameter in toxicological studies.
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