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. 1977 Apr;73(1):257-60.
doi: 10.1083/jcb.73.1.257.

Distribution of bicarbonate-stimulated ATPase in rat intestinal epithelium

C H van Os, A K Mircheff, E M Wright

Distribution of bicarbonate-stimulated ATPase in rat intestinal epithelium

C H van Os et al. J Cell Biol. 1977 Apr.

Abstract

This study reports on the distribution of bicarbonate-stimulated ATPase in rat intestinal epithelial cells. Brush-border membranes and basolateral membranes were separated from each other and from mitochondrial and other intracellular membranes by differential and density gradient centrifugation. Bicarbonate-sensitive ATPase activity followed the mitochondrial marker succinic dehydrogenase closely throughout all the centrifugation steps. The low HCO3--ATPase activity in purified brush-border and basolateral plasma membranes could be accounted for quantitatively by the small mitochondrial contamination. Consequently, there are no grounds for postulating that this enzyme has a direct role in H+ or HCO3- transport across the rat small intestine.

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References

    1. J Membr Biol. 1976 Sep 17;28(4):309-33 - PubMed
    1. Biochim Biophys Acta. 1965 Sep 20;105(3):472-82 - PubMed
    1. Biochim Biophys Acta. 1971 Jul 6;241(1):49-56 - PubMed
    1. Enzyme. 1971;12(4):499-507 - PubMed
    1. Biochim Biophys Acta. 1972 Jul 3;274(1):189-200 - PubMed

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