Syndecan-1, a cell-surface proteoglycan, changes in size and abundance when keratinocytes stratify

Abstract

In epidermis, keratinocytes in the basal cell layer differentiate, lose their attachment to the underlying extracellular matrix, and form extensive intercellular adhesions as they stratify. The alterations in cell-matrix and cell-cell adhesion required for keratinocyte stratification result from changes in the expression of numerous adhesion molecules. Syndecan-1, a member of a family of cell-surface proteoglycans, is known to bind cells to interstitial matrix. Syndecan-1 localizes to specific layers of mouse epidermal keratinocytes; its expression is modest in the basal layer, heavy in the suprabasal layers, but absent from the most superficial, terminally differentiated layers. This layer-specific difference suggests that syndecan-1 expression changes with keratinocyte differentiation. To assess this hypothesis, syndecan-1 expression was evaluated before and after calcium-induced stratification and differentiation. Cells growing as an unstratified monolayer express a higher molecular mass form of syndecan-1 than do stratified cells (modal relative mass of 160 kD versus 110 kD). This structural difference is due to larger and more heparan sulfate chains on syndecan-1 from monolayer cells. In addition, the amount of cell-surface syndecan-1 changes with stratification; stratified cultures show approximately 2.5 times more syndecan-1 per cell than do unstratified cultures, but do not significantly change the level of syndecan-1-specific mRNA. Thus, the structure and amount of syndecan-1 may be regulated to meet the changing adhesive requirements of stratifying keratinocytes.