[Index of fibrinolysis with new fibrin plate (author's transl)]

Abstract

Plasminogen-free fibrin plate (fP) which was made from treated commerical bovine fibrinogen with Lysine-Sepharose was developed in our laboratory. This new fibrin plate showed the following specificities. a) This new fibrin plate did not show any lysis with high amount of streptokinase and Urokinase (10,000 u/ml and 500 u/ml). b) The concentrations of its substrate was the same as standard plate (SP) and its substrate was not denatured compared with heated plate (HP). c) The activity of plasmin can be measured quantitatively on fP and linear correlation between plasmin units and lysis area was showen. d) This procedure of new fibrin plate was easy and simple and could be applicable to the materials of other species, i.e., human, rabbit and porcine. With the use of two kinds of bovine fibrin plate (SP and fP), activation of fibrinolysis of human plasma, euglobulin and plasminogen induced by SK and UK was investigated and each correlation ship between sample and activator was studied statistically. From these results, "Index of fibrinolysis" meaning of fibrinolytic components such as plasmin, plasminogen, activator, proactivator, anti-activator and anti-plasmin were indicated. Indeed, these index of fibrinolysis were calculated from the lysis area of plasma+SK, Eug.+SK and Eug.+UK by each formula and index obtained from some physiological and pathological condition showed us many new information about fibrinolysis.