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. 1963 Nov;3(6):469-78.
doi: 10.1016/s0006-3495(63)86832-0.

THE REACTIONS OF HEMOGLOBIN IN VARIOUS MEDIA

E ACKERMAN, R L BERGER, W L BLAIR, G K STROTHER

THE REACTIONS OF HEMOGLOBIN IN VARIOUS MEDIA

E ACKERMAN et al. Biophys J. 1963 Nov.

Abstract

The rate constants for the association of oxygen and of carbon monoxide with reduced hemoglobin and for the dissociation of oxygen from hemoglobin were studied in a variety of media. All three of these rates were essentially independent of diffusion rate and dielectric constant throughout a wide range. These results are very different from those found for other heme proteins whose rates are diffusion-controlled in very viscous solvents.The interpretation of the average measured rates described herein in terms of the four rate constants discussed by Gibson and Roughton indicates that our measurements reflect most strongly the constants for the first reacting molecule in each case. For the purposes of these reactions, the reactants may be regarded as neutral molecules.

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References

    1. Proc R Soc Lond B Biol Sci. 1955 Mar 15;143(912):310-34 - PubMed
    1. J Physiol. 1955 Jul 28;129(1):65-89 - PubMed
    1. Am J Physiol. 1959 Mar;196(3):517-9 - PubMed

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