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. 1992 Sep 11;20(17):4451-5.
doi: 10.1093/nar/20.17.4451.

Stoichiometry of the Cre recombinase bound to the lox recombining site

A Mack  1 , B Sauer, K Abremski, R Hoess
Affiliations
Free PMC article

Stoichiometry of the Cre recombinase bound to the lox recombining site

A Mack et al. Nucleic Acids Res. .
Free PMC article

Abstract

The site-specific recombinase Cre from bacteriophage P1 binds and carries out recombination at a 34 bp lox site. The lox site consists of two 13 bp inverted repeats, separated by an 8 bp spacer region. Both the palindromic nature of the site and the results of footprinting and band shift experiments suggest that a minimum of two Cre molecules bind to a lox site. We report here experiments that demonstrate the absolute stoichiometry of the Cre-lox complex to be one molecule of Cre bound per inverted repeat, or two molecules per lox site.

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