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. 1963 Oct:32:205-8.
doi: 10.1016/0014-4827(63)90093-4.

A METHOD FOR IN VITRO CULTIVATION OF MOUSE OVA FROM TWO-CELL TO BLASTOCYST

A METHOD FOR IN VITRO CULTIVATION OF MOUSE OVA FROM TWO-CELL TO BLASTOCYST

R L BRINSTER. Exp Cell Res. 1963 Oct.
No abstract available

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Figures

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Fig. 1.—Culture dish with four microdroplets of media under light-weight paraffin oil. Approxinately original size. Fig. 2.—Two-cell mouse ova at beginning of in vitro culture. ×60. Fig. 3.—Eight-cell stage. Developed from two-cell mouse ova cultured in vitro for 24 hr. ×60. Fig. 4.—Morulae and early blastocysts. Developed from two-cell ova cultured in vitro for 48 hr. ×60. Fig. 5.—Late blastocyst escaping from zona pellucida. Developed from two-cell ova cultured in vitro for 72 hr. ×60. Fig. 6.—Late blastocyst completely free of zona. Developed from two-cell ova cultured in vitro for 72 hr. ×120.

References

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