Review
doi: 10.1016/0163-7258(92)90056-6.
Serum-free cell culture
Affiliations
- PMID: 1409851
- DOI: 10.1016/0163-7258(92)90056-6
Item in Clipboard
Review
Serum-free cell culture
Pharmacol Ther.
1992.
Abstract
Cell culture is one important tool when studying cellular functions and molecular biology. It is also a basic method in most virological investigations. Serum has been an obligatory component in most cell culture media. During the last decades serum-free, chemically defined media have been developed, that are supplemented with a number of substances with specific cellular activities. The main developments of defined media are presented. Examples are given of investigations with different cell types.
Similar articles
-
Alternatives to the use of fetal bovine serum: serum-free cell culture.ALTEX. 2003;20(4):275-81. ALTEX. 2003. PMID: 14671707
-
Safety issues of animal products used in serum-free media.Dev Biol Stand. 1999;99:167-80. Dev Biol Stand. 1999. PMID: 10404888 Review.
-
[Serum-free culture--a theoretical note].Hum Cell. 1991 Jun;4(2):87-92. Hum Cell. 1991. PMID: 1958635 Review. Japanese.
-
Differences in the glycosylation profile of a monoclonal antibody produced by hybridomas cultured in serum-supplemented, serum-free or chemically defined media.Biotechnol Appl Biochem. 2007 Jun;47(Pt 2):113-24. doi: 10.1042/BA20060216. Biotechnol Appl Biochem. 2007. PMID: 17250495
-
Development of serum-free media for lepidopteran insect cell lines.Methods Mol Biol. 2007;388:155-86. doi: 10.1007/978-1-59745-457-5_8. Methods Mol Biol. 2007. PMID: 17951770
Cited by
-
Functional Assessment for Clinical Use of Serum-Free Adapted NK-92 Cells.Cancers (Basel). 2019 Jan 10;11(1):69. doi: 10.3390/cancers11010069. Cancers (Basel). 2019. PMID: 30634595 Free PMC article.
-
Development of a modified serum-free medium for Vero cell cultures: effects of protein hydrolysates, l-glutamine and SITE liquid media supplement on cell growth.Cytotechnology. 2021 Oct;73(5):683-695. doi: 10.1007/s10616-020-00450-3. Epub 2021 Aug 11. Cytotechnology. 2021. PMID: 34629745 Free PMC article.
-
Best practices for media selection for mammalian cells.In Vitro Cell Dev Biol Anim. 2017 Sep;53(8):673-681. doi: 10.1007/s11626-017-0186-6. Epub 2017 Jul 19. In Vitro Cell Dev Biol Anim. 2017. PMID: 28726187 Review.
-
Heterogeneic modulation of malignant behavior in human glioma cells in defined and serum-containing media.In Vitro Cell Dev Biol Anim. 1996 Mar;32(3):159-66. doi: 10.1007/BF02723681. In Vitro Cell Dev Biol Anim. 1996. PMID: 8925138
-
Hyperplastic Human Macromass Cartilage for Joint Regeneration.Adv Sci (Weinh). 2023 Sep;10(26):e2301833. doi: 10.1002/advs.202301833. Epub 2023 Jul 3. Adv Sci (Weinh). 2023. PMID: 37395375 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
