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. 1964 Jan;7(1):72-81.

THE EFFECT OF PH AND IONIC STRENGTH ON THE REACTION BETWEEN ANTI-D AND ERYTHROCYTES

N C HUGHES-JONES, B GARDNER, R TELFORD

THE EFFECT OF PH AND IONIC STRENGTH ON THE REACTION BETWEEN ANTI-D AND ERYTHROCYTES

N C HUGHES-JONES et al. Immunology. 1964 Jan.

Abstract

The effect of pH and ionic strength on the reaction between anti-D and erythrocytes was investigated using 131I-labelled antibody. The following observations were made.

1. The association constant of the reaction was influenced by the pH of the suspending medium. The highest values were obtained in the pH range 6.0–8.0. The association constant was considerably reduced above and below these values.

2. There was considerable heterogeneity of the rate of dissociation of the antigen—antibody complex at pH values below 6.0

3. The antibody was separated into fractions which, at neutral pH, had different rate constants for dissociation.

4. The rate of association between anti-D and erythrocytes was greatly increased by a reduction in the ionic strength of the suspending medium.

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References

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