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. 1964 May 29;144(3622):1147-8.
doi: 10.1126/science.144.3622.1147.

N-ETHYLMALEIMIDE INHIBITION OF HORSESHOE CRAB HEMOCYTE AGGLUTINATION

F T BRYAN, C W ROBINSON Jr, C G GILBERT, R D LANGDELL

N-ETHYLMALEIMIDE INHIBITION OF HORSESHOE CRAB HEMOCYTE AGGLUTINATION

F T BRYAN et al. Science. .

Abstract

The sulfhydryl inhibitor N-ethylmaleimide (NEM) inhibits the agglutination of horseshoe crab hemocytes. The inhibitory action is neutralized by adding cysteine to the NEM before it is mixed with the hemolymph. This new method for arresting horseshoe crab hemocyte agglutination suggests the participation of sulfhydryl groups in the process.

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