MITOTICALLY SYNCHRONIZED MAMMALIAN CELLS: A SIMPLE METHOD FOR OBTAINING LARGE POPULATIONS
- PMID: 14148440
- DOI: 10.1126/science.144.3622.1152
MITOTICALLY SYNCHRONIZED MAMMALIAN CELLS: A SIMPLE METHOD FOR OBTAINING LARGE POPULATIONS
Abstract
Eliminating most of the ionic calcium from growth medium does not affect cell growth, but it allows simple and preferential detachment of HeLa cells in mitosis from nearly confluent monolayers.
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