Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1964 May;47(5):975-86.
doi: 10.1085/jgp.47.5.975.

EFFECT OF DETERGENT ON ELECTRICAL PROPERTIES OF SQUID AXON MEMBRANE

U KISHIMOTO, W J ADELMAN Jr

EFFECT OF DETERGENT ON ELECTRICAL PROPERTIES OF SQUID AXON MEMBRANE

U KISHIMOTO et al. J Gen Physiol. 1964 May.

Abstract

The effects of detergents on squid giant axon action and resting potentials as well as membrane conductances in the voltage clamp have been studied. Anionic detergents (sodium lauryl sulfate, 0.1 to 1.0 mM; dimethyl benzene sulfonate, 1 to 20 mM, pH 7.6) cause a temporary increase and a later decrease of action potential height and the value of the resting potential. Cationic detergent (cetyl trimethyl ammonium chloride, 6 x 10(-5)M or more, pH 7.6) generally brings about immediate and irreversible decreases in the action and resting potentials. Non-ionic detergent (tween 80, 0.1 M, pH 7.6) causes a slight reversible reduction of action potential height without affecting the value of the resting potential. Both anionic and cationic detergents generally decrease the sodium and potassium conductances irreversibly. The effect of non-ionic detergent is to decrease the sodium conductance reversibly, leaving the potassium conductance almost unchanged.

PubMed Disclaimer

References

    1. J Gen Physiol. 1961 Sep;45:93-103 - PubMed
    1. Jpn J Physiol. 1956 Jun 15;6(2):137-49 - PubMed
    1. J Gen Physiol. 1959 Mar 20;42(4):793-802 - PubMed
    1. J Gen Physiol. 1960 Sep;44:123-67 - PubMed
    1. J Physiol. 1952 Apr;116(4):473-96 - PubMed