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. 1964 Dec 11;146(3650):1476-7.
doi: 10.1126/science.146.3650.1476.

BONE CELLS: BIOCHEMICAL AND BIOLOGICAL STUDIES AFTER ENZYMATIC ISOLATION

W A PECK, S J BIRGE Jr, S A FEDAK

BONE CELLS: BIOCHEMICAL AND BIOLOGICAL STUDIES AFTER ENZYMATIC ISOLATION

W A PECK et al. Science. .

Abstract

Short-term incubation of rat calvaria in buffered crude collagenase permitted the isolation of morphologically intact cells that absorb vital dyes, contain alkaline phosphatase, and multiply in tissue culture. Freshly harvested cells were similar to whole bone segments in aerobic glucose metabolism.

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