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. 1992 Mar;2(2):195-202.
doi: 10.1007/BF01875530.

A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules

B T Farmer 2nd  1 , R A Venters, L D Spicer, M G Wittekind, L Müller
Affiliations

A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules

B T Farmer 2nd et al. J Biomol NMR. 1992 Mar.

Abstract

A 3D optimized, refocused HNCA experiment is described. It is demonstrated to yield a dramatic increase in sensitivity when applied to [13C, 15N]-labeled human carbonic anhydrase II, a 29-kDa protein. The reasons for the gain in sensitivity are discussed, and 3 distinct areas for further development are indicated.

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References

    1. J Biomol NMR. 1991 May;1(1):99-104 - PubMed
    1. Biochemistry. 1990 May 15;29(19):4659-67 - PubMed

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