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. 1976 Aug;32(2):311-4.
doi: 10.1099/0022-1317-32-2-311.

The use of tris-HCl buffer for inoculation of tomato protoplasts with tobacco mosaic virus

F Motoyoshi, N Oshima

The use of tris-HCl buffer for inoculation of tomato protoplasts with tobacco mosaic virus

F Motoyoshi et al. J Gen Virol. 1976 Aug.

Abstract

When tris-HCL was used as the buffer for inoculation of tomato protoplasts with tobacco mosaic virus, a greater proportion became infected than when phosphate was used. Using 0-05 M-tris-HCl buffer, pH 8-0, in the presence of poly-L-ornithine or poly-D-lysine, 50 to 80 % infection was obtained.

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