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. 1964 Nov;17(6):676-9.
doi: 10.1136/jcp.17.6.676.

ESTIMATION OF SERUM HAEMOGLOBIN-BINDING CAPACITY (HAPTOGLOBIN) ON SEPHADEX G.100

A P RATCLIFF, J HARDWICKE

ESTIMATION OF SERUM HAEMOGLOBIN-BINDING CAPACITY (HAPTOGLOBIN) ON SEPHADEX G.100

A P RATCLIFF et al. J Clin Pathol. 1964 Nov.

Abstract

A method is described for the estimation of serum haptoglobin as ;haemoglobin-binding capacity'. The method relies on column chromatographic separation of the haemoglobin/haptoglobin complex from excess added free haemoglobin on the dextran gel Sephadex G.100. The method is simple and reproducible, and correlates well with another method of estimating haemoglobin-binding capacity over a wide range of values.

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