Restoration of detergent-inactivated adenosine triphosphatase activity of human prostatic fluid with concanavalin A
- PMID: 142513
- DOI: 10.1016/0005-2744(77)90078-x
Restoration of detergent-inactivated adenosine triphosphatase activity of human prostatic fluid with concanavalin A
Abstract
A stimulation by concanavalian A OF Mg2+ -and Ca2+-dependent ATPase (ATP phosphohydrolase, EC 3.6.1.3) of human prostatic fluid has been observed after the enzyme system had been inactivated by a detergent such as 0.05% deoxycholate. The concanavalin A effect was specific since the positive effect was abolished in the presence o alpha-methyl-D-mannoside. Furthermore, the positive effect of concanavalin A was obtained with a low lectin concentration, equal to the concentration reported for optimal stimulation of other membrane enzymes.
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