Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1965 Jan;89(1):84-8.
doi: 10.1128/jb.89.1.84-88.1965.

CHEMICAL AND IMMUNOLOGICAL COMPOSITION OF SURFACE STRUCTURES OF LISTERIA MONOCYTOGENES

CHEMICAL AND IMMUNOLOGICAL COMPOSITION OF SURFACE STRUCTURES OF LISTERIA MONOCYTOGENES

J W OSEBOLD et al. J Bacteriol. 1965 Jan.

Abstract

Osebold, John W. (University of California, Davis), Ole Aalund, and Clarence E. Chrisp. Chemical and immunological composition of surface structures of Listeria monocytogenes. J. Bacteriol. 89:84-88. 1965.-A proteinlike surface substance was demonstrated on Listeria monocytogenes when an explanation was sought for the inagglutinability of some somatic antigens. The serological behavior of live bacteria and organisms subjected to heat, formalin, and trypsin was compared. The agglutination-inhibiting phenomenon was most pronounced with heat-killed (100 C) antigens. Trypsinization eliminated inagglutinability and increased sensitivity. Substances released by the enzyme had an ultraviolet-absorption peak at 260 mmu and showed a spot on paper chromatograms compatible with polypeptide. Inagglutinable cells combined with antibody because they could readily absorb antibodies from serum. After reaction with anti-Listeria serum, inagglutinable cells could be agglutinated by the addition of antiglobulin serum. It was hypothesized that heat inactivation of cells denatured the proteinaceous surface layer which interferred with the formation of a visible agglutination product but did not eliminate antigen-antibody reaction.

PubMed Disclaimer

References

    1. Medizinische. 1956 Jul 14;7(27-28):977-82 - PubMed
    1. J Clin Invest. 1958 Feb;37(2):272-84 - PubMed
    1. Z Hyg Infektionskr. 1955;141(1):15-24 - PubMed
    1. Br J Exp Pathol. 1954 Apr;35(2):166-80 - PubMed
    1. Can J Microbiol. 1956 May;2(3):220-31 - PubMed

LinkOut - more resources