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. 1992 Nov;58(5):977-80.
doi: 10.1016/s0015-0282(16)55445-4.

Freezing cocultured human blastocysts

Y Ménézo  1 , B Nicollet, N Herbaut, D André
Affiliations
Free article

Freezing cocultured human blastocysts

Y Ménézo et al. Fertil Steril. 1992 Nov.
Free article

Abstract

Objective: To determine the possibility of obtaining good pregnancy rates (PRs) after freezing and thawing cocultured blastocysts.

Design: Human blastocysts were frozen first according to a protocol available from literature. Two other protocols including the addition of glycerol were designed to improve the results.

Setting: All the patients were under clinical management at the Institut Rhonalpin pour l'Etude de la Reproduction Humaine in Lyons, France.

Patients: Patients involved in the in vitro fertilization program have had their supernumerary embryos frozen according to the three protocols presented here.

Main outcome measures: Embryo recovery after freezing and thawing. Clinical and ongoing pregnancies after embryo transfer (ET).

Results: A protocol including sucrose addition and reduction of steps in the preparation of the blastocysts for freezing gave us a 21% PR per transfer (15 ongoing) of 101 transfers (106 thawings).

Conclusions: Freezing of cocultured human blastocysts allow good PRs. This can represent an alternative for repeated failures of ETs at early stages.

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