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Comparative Study
. 1992 Oct 1;76(1-2):77-81.
doi: 10.1111/j.1574-6968.1992.tb05443.x.

Comparison of the nucleotide sequence and development of a PCR test for the epsilon toxin gene of Clostridium perfringens type B and type D

H L Havard  1 , S E Hunter, R W Titball
Affiliations
Comparative Study

Comparison of the nucleotide sequence and development of a PCR test for the epsilon toxin gene of Clostridium perfringens type B and type D

H L Havard et al. FEMS Microbiol Lett. .

Abstract

The sequence of the epsilon toxin gene of Clostridium perfringens type D was determined and compared with that of the previously reported type B sequence. It showed two nucleotide changes in the open reading frame, giving rise to one amino acid substitution. The promoter sequences were not homologous, and different putative -35 and -10 regions have been identified in each. The sequence information was used to develop PCR primers which were specific for the epsilon toxin gene. The utility of this system for identifying type B or D strains of C. perfringens was demonstrated.

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