doi: 10.1093/oxfordjournals.jbchem.a131670.
A simple method for the isolation of actin from myxomycete plasmodia
- PMID: 142766
- DOI: 10.1093/oxfordjournals.jbchem.a131670
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A simple method for the isolation of actin from myxomycete plasmodia
J Biochem.
1977 Jul.
Free article
Abstract
A new, simple method for the isolation of actin from myxomycete plasmodia has been developed. Plasmodium myosin B was incubated at 55 degrees C for 15 min in the presence of ATP or was treated with 90% acetone. By this treatment myosin was denatured completely. Actin was then extracted with a dilute ATP and cysteine solution from the heat- or acetone-treated myosin B. The method is simple and almost pure actin was obtained in high yield. The purified G-actin polymerized to F-actin on addition of 0.1 M KCl or 2 mM MgCl2. The viscosity of the purified F-actin was 8-10 dl/g. The F-actin activated muscle myosin ATPase, and actomyosin synthesized from the F-actin and muscle myosin showed superprecipitation on addition of ATP.
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