A SENSITIVE METHOD FOR THE DETERMINATION OF CARBOXYHAEMOGLOBIN IN A FINGER PRICK SAMPLE OF BLOOD
- PMID: 14278801
- PMCID: PMC1008260
- DOI: 10.1136/oem.22.2.139
A SENSITIVE METHOD FOR THE DETERMINATION OF CARBOXYHAEMOGLOBIN IN A FINGER PRICK SAMPLE OF BLOOD
Abstract
About 0·01 ml. of blood taken from a finger prick is dissolved in 10 ml. of 0·04% ammonia solution. The solution is divided into two halves, and oxygen is bubbled through one half to convert any carboxyhaemoglobin into oxyhaemoglobin. The spectra of the two halves are then compared in a spectrophotometer, and the difference between them is used to estimate the carboxyhaemoglobin content of the blood either graphically or by calculation from a simple formula. Calibration is simple and need only be done once. A sample of blood can be analysed in about 20 minutes, which includes the time to collect the sample. The method is sensitive enough to be used for the analysis of solutions of blood containing less than 1% carboxyhaemoglobin.
Similar articles
-
Spectrophotometric determination of oxygen saturation of hemoglobin in the presence of carboxyhemoglobin.Scand J Clin Lab Invest. 1965;17(5):491-500. Scand J Clin Lab Invest. 1965. PMID: 5839889 No abstract available.
-
THE DETERMINATION AND SIGNIFICANCE OF LOW BLOOD CARBOXYHAEMOGLOBIN LEVELS.Med Sci Law. 1964 Apr;4:98-107. doi: 10.1177/002580246400400204. Med Sci Law. 1964. PMID: 14138587 No abstract available.
-
[THE STUDY ON THE SKIN PERMEABILITY OF CARBON MONOXIDE AND MEASUREMENT OF CARBOXYHAEMOGLOBIN IN BLOOD].Nihon Hoigaku Zasshi. 1963 Jul;17:314-26. Nihon Hoigaku Zasshi. 1963. PMID: 14043117 Japanese. No abstract available.
-
Spectrophotometry of hemoglobin and hemoglobin derivatives.Adv Clin Chem. 1983;23:199-257. doi: 10.1016/s0065-2423(08)60401-1. Adv Clin Chem. 1983. PMID: 6398614 Review. No abstract available.
-
Determination of hemoglobin and its derivatives.Adv Clin Chem. 1965;8:141-87. doi: 10.1016/s0065-2423(08)60414-x. Adv Clin Chem. 1965. PMID: 5321443 Review. No abstract available.
Cited by
-
Comparison of the inhalation toxicity of kretek (clove cigarette) smoke with that of American cigarette smoke. II. Fourteen days, exposure.Arch Toxicol. 1990;64(7):515-21. doi: 10.1007/BF01971829. Arch Toxicol. 1990. PMID: 2073125
-
An experimental study of the pathogenesis of Grinker's myelinopathy in carbon monoxide intoxication.Acta Neuropathol. 1983;59(3):200-6. doi: 10.1007/BF00703204. Acta Neuropathol. 1983. PMID: 6845982 No abstract available.
-
Letter: Blood carboxyhaemoglobin levels in smokers.Br Med J. 1973 Dec 22;4(5894):740. doi: 10.1136/bmj.4.5894.740. Br Med J. 1973. PMID: 4776897 Free PMC article. Clinical Trial. No abstract available.
-
Blood carbon monoxide levels as a function of daily cigarette consumption and physical activity.Br J Ind Med. 1976 May;33(2):123-5. doi: 10.1136/oem.33.2.123. Br J Ind Med. 1976. PMID: 1276092 Free PMC article. No abstract available.
-
The relationship between zinc protoporphyrin (ZPP) and "free" erythrocyte protoporphyrin (FEP) in lead-exposed individuals.Int Arch Occup Environ Health. 1980;47(2):165-77. doi: 10.1007/BF00716375. Int Arch Occup Environ Health. 1980. PMID: 7440002
References
-
- J Appl Physiol. 1964 May;19:510-5 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources