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. 1965 Apr;8(4):406-10.

ANALYSIS OF ANTIGEN-ANTIBODY COMPLEXES BY IMMUNOELECTROPHORESIS

F MILGROM, Z M TUGGAC, W A CAMPBELL

ANALYSIS OF ANTIGEN-ANTIBODY COMPLEXES BY IMMUNOELECTROPHORESIS

F MILGROM et al. Immunology. 1965 Apr.

Abstract

Immune complexes composed of two insoluble and two soluble antigens and their corresponding antibodies of rabbit origin were separated by electrophoresis in agar gel performed at 56°. Antibodies could then be demonstrated in the cathodal part of the electrophoretic field; they formed precipitation lines with goat antiserum to rabbit serum. The separation of soluble antigens could be demonstrated by the precipitation reaction with their corresponding antisera.

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References

    1. Nature. 1958 Jul 19;182(4629):189-90 - PubMed
    1. J Immunol. 1963 Oct;91:438-47 - PubMed
    1. Immunology. 1962 Jan;5:46-66 - PubMed

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